For the purification of insulin, YMC have specifically developed a new reversed phase material. This new phase addresses the specific needs of insulin purification which results in up to 40
Next generation RP for peptide purification In cooperation with two independent insulin manufacturers YMC developed a new phase for insulin purification. The real-life data demonstrate the great potential for the optimisation of existing insulin processes. The perfor- mance of this new phase exceeded the expectations of the cooperating partners. In fact, the loading can be up to 100
Screening results for a loading of 50 mg insulin Purification of insulin In summary, the YMC-Triart Prep Bio200 C8 phase shows the highest performance, resulting in the greatest cost efficiency and productivity. The isolation of 100 kg insulin with a purity of 99.5
Conclusions
In cooperation with insulin manufacturer, real insulin samples were used to perform a comprehensive screening and process optimisation study. The screening of different stationary phases provided a profound data basis. It allowed an extensive a cost estimation for 100 kg purified insulin. A linear scale up to a 60 cm ID column was chosen as a realistic scenario. The target for the process is to produce insulin with a purity of 99.5
In a detailed case study it was shown that YMC-Triart Prep Bio200 C8 phase delivers up to 40
