MICHAEL KOGANOV*, LI ZHANG, ARTYOM DUEV, OLGA DUEVA-KOGANOV, XIAOWEN HOU
*Corresponding author
Akzo Nobel Surface Chemistry
23 Snowden Avenue, Ossining, NY10562, U.S.A.

Abstract

Novel amphiphilic ingredients and Recentia^® CS (Camellia Sinensis Flower/Leaf/Stem Juice) were produced from living Tea Plant (Camellia sinensis) by Zeta Fraction™ technology and tested to determine their safety and efficacy in in vitro bioassays, e.g. scavenging of free radicals, inhibition of proteases, chemokines and cytokines. Potency of the amphiphilic ingredients, which are complex mixtures of compounds, at inhibiting Sodium Dodecyl Sulfate-induced Interleukin-1α in human epidermal keratinocytes is superior to 2-acetoxybenzoic acid, a well-known anti-inflammatory compound. The biological activities of the test articles are comprehensively addressing the interrelated pathways involved in skin inflammation and ageing. Our data suggest that while activities of Camellia sinensis preparations are commonly attributed to Catechins, especially Epigallocatechin gallate, the beneficial effects may be actually related to combined and/or synergistic activities, including those of compounds comprising over two-thirds of the novel ingredient composition not characterizable as Catechins or Theaflavins. These findings demonstrate that Zeta Fraction™ technology allows more comprehensive capture and efficacious utilization of the desired activities of Camellia sinensis. The amphiphilic ingredients and Recentia^® CS can be used alone or simultaneously in various delivery systems, providing formulators with the opportunity to explore the bioactive synergies of natural complexes and develop finished goods with superior efficacy.

INTRODUCTION

Tea Plant (Camellia sinensis) contains a wide spectrum of biologically active substances and provides various preventive and therapeutic benefits (1-7). Traditional manufacturing produces four major types of teas: black, oolong, green and white. Increased processing changes chemical compositions of teas and decreases therapeutic benefits (8-9). Novel Zeta Fraction™ technology enables effective utilization of underexplored potential of living plants while protecting the integrity of molecular architecture existing in plant living cells. This technology produces plant-derived bioactive complexes with greater diversity and complexity of constituents than traditional solvent ... ...