CIS display, a DNA-based in vitro selection technology for therapeutic peptides
PASCALE MATHONET1, AVGOUSTA IOANNOU2, JASON BETLEY2, CHRIS ULLMAN1*
*Corresponding author
1. Isogenica Ltd Chesterford Research Park Little Chesterford, CB10 1XL, United Kingdom
2. Illumina Cambridge Ltd Chesterford Research Park Little Chesterford, CB10 1XL, United Kingdom
Abstract
CIS display is a DNA-based in vitro display technology that enablesthe display and selection of peptides and proteins from extremelylarge libraries. The system uses a natural process to couple thephenotype and genotype and allow selection or maturation of highaffinity peptide binders against targets. In this article, we review the CISdisplay technique and its integration with next-generation sequencingand bioinformatics to provide a data-rich output to facilitate rationaldesign of biologics and small molecules and also for intellectualproperty protection.
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