YMC – Peptide and Oligonucleotide Purification with Reversed Phase and Ion Exchange Chromatography
New Focus on Biomolecules
Peptides and oligonucleotides are becoming increasingly important as biopharmaceuticals. The broad functional spectrum of these molecules offers a wide range of pharmaceutical applications. On the other hand, their variety in chemical and physical properties as well as impurities with similar characteristics complicate the reliable and safe production. For their purification, (ion-pair-) reversed phase (IP-RP) and ion exchange (IEX) chromatography are ideal techniques. The charged oligonucleotide backbone is excellently suited for IEX but on the other hand cannot interact sufficiently with the reversed stationary phase. Therefore ion-pair reagents with a hydrophobic group are required. They act as mediators between the charged target and the hy ... ...
